Eugenia Dumitrescu
Banat’s University of Agriculture and Veterinary Medicine, Romania
Title: The anti-proliferative activity of Coriandrum sativum alcoholic extract on HCT-116 and MCF-7 cell lines
Biography
Biography: Eugenia Dumitrescu
Abstract
Statement of the Problem: It is known that vegetal extracts can generate positive responses in stages of several patho-processes. The anti-oxidant; protection against DNA damage and cancer activities of Coriandrum sativum were intensely studied in the last decade. Our research envisaged the polyphenolic compounds’ structure and the anti-proliferative biologic activity of C. sativum 10 % alcoholic extract against HCT-116 and MCF-7 cell lines.
Methodology: The C. sativum extract was obtained respecting the Romanian Pharmacopoeia 10th edition, the plant being lyophilized (with Ilshin Kryptonstraat 11, 6718WR EBE lyophilisator to -55 oC, 5 mTorr pressure and 24 hours lyophilisation time). The polyphenols were determined by LC-MS and the in vitro evaluation effects by the MTT proliferation test, using HCT116 (colorectal carcinoma) and MCF7 (mammary adenocarcinoma). The cells were seed as: 2×104 (MCF7) and 1×104 (HCT) in 96 well plates. The lyophilized extracts were suspended in specific culture medium being obtained a 300 mg/mL C. sativum stock solution. From this, different test concentrations were prepared by dilution (300, 200, 100 and respectively 50 mcg/mL).
Result & Conclusion: As a following, after 24 hours from the exposure, using HCT-116 and MCF-7 cell lines it was observed that the cellular proliferation reduced, this being correlated to dose and the alterations of cell morphology to the groups studied; to great extract doses, apoptotic and necrotic alterations were observed, both for HCT and MCF cells; the IC50, representing concentration to which a marker substance is reducing the tissues viability with 50% after a fixed time exposure period wasn’t observed for the cell lines used in this test; the chromatographic analysis of C. sativum alcoholic extract evidenced the presence of the polyphenolic compounds, the greatest concentrations were ascertained for epicatechin (77.083 mcg/mL) and rutin (30.279 mcg/mL), substances with known hard anti-oxidant proprieties.