Day 2 :
National Research Institute, Poland
Time : 09:30-10:30
Lidia Zapór works in the Department of Chemical, Aerosol and Biological Hazards in the Central Institute for Labour Protection – National Research Institute (CIOP-PIB). She is a head of Laboratory of Toxicology. The main area of her professional interest are problems of human in the working environment and the toxicity of chemical substances as well as methods of estimating the toxicity of substances in vitro. She was also engaged in preparation of documentation of maximum allowable levels of occupational exposure and characteristics of hazardous substances in the work of the Interdepartmental Committee mandated with updating and verification of the Threshold Limit Values (TLVs) for agents harmful to human health.
Statement of the Problem: Nanostructured molybdenum trioxide (MoO3-NPs) is promising material in many applications: in coatings, plastics, textiles, pigments, lubricants, ceramics and glass production, as antimicrobial agents, and for the detection of dopamine in pharmaceutical and clinical preparations. In recent years, it was noted that in the case of nanomaterials should carefully evaluate the risks of their use, as they may pose a health risk. The objective of this study was to assess the cytotoxic activity of MoO3-NPs in human pulmonary cells.
Methodology: The cytotoxicity of MoO3-NPs was assessed on the alveolar carcinoma epithelial cells (A549) and normal bronchial epithelium cells (BEAS-2B) after short, and long-term time of exposure. Cytotoxicity studies included the effect of MoO3-NPs on cell viability, cell membrane integrity (NRU assay), mitochondrial metabolic activity (MTT assay) and the ability of the cells to proliferation (Clonogenic assay).
Findings: MoO3-NPs induced a dose- and time-related negative effect on the viability of both kids of the cells in the cytotoxic doses range 50 - 300 µg/ml, depending on cytotoxicity endpoint. In long-term exposure (7 day), MoO3-NPs at concentrations about 100 µg/ml impaired proliferation, implying their potential chronic toxicity. A549 cells were less sensitive than BEAS-2B one, to all measurement parameters.
Conclusion & Significance: The sensitivity of BEAS-2B cells to MoO3-NPs is of particular concern. These cells form a defense line of the body against the penetration of particles into lungs. Inhibition of the ability of BEAS-2B cells to proliferate under the influence of MoO3-NPs may be an unfavourable phenomenon for predicting their long-term effects of exposure.
Funding. This paper has been based on the results of a research task II.N11A. carried out within the scope of the fourth stage of the National Programme Improvement of safety and working conditions partly supported in 2017–2019 — within the scope of research and development — by the Ministry of Science and Higher Education/National Centre for Research and Development. The CIOP-PIB is the Programmes main co-ordinator.
Kaohsiung Medical University, Taiwan
Dr. Ming-Tsang Wu has completed his MD from Chung Shan Medical University in Taiwan and PhD from Harvard School of Public Health in the USA. He is a full professor in the Department of Public Health and the Director in Research Center for Environmental Medicine, Kaohsiung Medicine University, Taiwan. His major research interest is on the interactive effects of environmental and occupational exposures, genetic factors, and biomarkers on the health outcomes.
We are still exposed to low-dose melamine in daily-life environment, even after 2008 toxic milk food scandal. One of the main sources is the intake of melamine chemical from the migration of melamine-made tableware, when contacted with high-temperature soup/water. Our previous study has found that chronic low-dose melamine exposure is associated with the risk of renal stones in adults, but the data about the relationship between environmental melamine exposure and the risk of renal damage in humans is still lacking. In this talk, I will present our recent findings about that link from different susceptible populations and propose the mechanisms behind that.
Shobhit University, India
Dr. Somenath Ghosh has worked in the field of hormone biology and congenital disorders of human physiology and involved in this area since 2009 both as doctoral and Post-Doctoral fellows. Presently he is involved in research where he is trying to investigate the possible amelioration of osteoporosis and other fragile Bone Diseases by biodegradable Poly-L-Lactic Acid-Melatonin (PLA-Mel-Nano-particles) in a CSIR funded project. He is serving Shobhit University, Gangoh, Saharanpur, India as an Assistant Professor. Apart from having numerous publications in journals of National and International repute, recently they were invited to contribute a “Book Chapter” in a Book entitled “Recent Advances in Food Processing and Technology” going to be published by Springer-Nature Publishing House, USA.
Statement of the Problem: Melatonin loaded PLA-Nano particles are nowadays important in biological system for it’s biodegradable nature in the mammalian system for systemtic/targetted drug delivery system. The aim of the present study is note the effect of Mel-PLA-Nano particles and toxicity in osteoprosis which is a lesser explored area of research.
Methodology & Theoretical Orientation: Different concentrations of commercially available melatonin and PLA were prepared by dissolving in dichloromethane (DCM). The final dried nano-paticles were stored at 4°C for future use and a part was used for structural analysis by Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Fourier Transform Infrared Spectroscopy (FTIR). Further, to evaluate the toxicity and immunological impact of nano-particles on rats; animals were divided into 2 groups (control and nano-particle treated, n=5/group) for 7 days. After treatment animals were sacrificed and blood, liver and kidney were collected. A fraction of blood was processed for assessment of cell mediated immune parameters (TLC, DLC and % LC) and remaining blood was centrifuged at 3000 x g at 4°C for 30 min. Plasma was seperated and used for evaluation of cytokines, (IL-2, IL-6, TNF-α, IFN-γ, IL-1β), makers of renal function test (urea, creatinine and BUN). Both plasma and tissue homogenates were used for estimations of AST, ALT, ACP and ALP. The nano-particle size was evaluated by SEM and TEM and was further confirmed by FTIR and in vitro controlled drug release efficasy.
Findings: We noted signifacntly high (p < 0.05) levels of cell mediated immune parameters (TLC, DLC and %LC) and cytokine parameters (IL-2 and TNF-α) upon nano-particle treatment. But rest of the parametrs were found to be significantly low (like IL-6, IL-1β, AST, ALT; p > 0.05) or un-affected (like IFN-γ, ACP and ALP).
Conclusion & Significance: From our preliminary study we may conclude that we have successfully synthesized Mel-PLA-nano-particles and their physilogical effects were non-toxic to the animal.