Gulrana Khuwaja
Jazan University, Saudi Arabia
Title: Mechanistic approach on the combined toxicity of selenium and tellurium in rat neuroendocrine centers
Biography
Biography: Gulrana Khuwaja
Abstract
Selenium and tellurium are widely used in so many industries including steel, rubber, electronic, ceramic, glass, zinc, arsenic, cadmium, aluminum, copper, tin, thallium, daylight lamps, electronics, photocells, silicon semiconductor devices, manufacture photographic exposure, solar cells, xerography, red or black glass, pigments in plastics, paints, dyes, enamels, inks, textiles, rubber, photographic emulsions, and petroleum (Taylor, 1996, Se-Te, 2013). Earlier Islam et al. (2002, 2004) and his group (Kaur et al., 2003 a, b) has reported its neurotoxicity on lipids and oxidative stress parameters in the brain. Extending the earlier work of Islam group, we further studied the toxicity of Se and Te on neuroendocrine centers in rats brain. This will be the first report of its kind. The male Wistar rats treated with 1/20th of LD50 of sodium tellurite (4.15 mg, orally), 0.3 mg/kg sodium selenite (i.p) and the half combination of these metals i.e., sodium tellurite (2.075, orally) + sodium selenite (0.15 mg/kg, i.p) for a period of 15 days. On day 16, the animals were sacrificed and brains were taken out to dissect the hypothalamus, pituitary and pineal gland. Each brain part was weight and homogenized in 10 mM Tris-HCl, pH 7.4 to give 5 % homogenate. The homogenate was used for the assay of lipid peroxidation and activity of SOD. The supernatant (15,000 g x 20 min at 4 °C) was used for the other enzymatic and non-enzymatic assays (GSH GPx, GR, GST, Catalase, Caspase-3, and Caspase- 9.