Katarzyna Miranowicz-Dzierżawska
National Research Institute, Poland
Title: Differences between cytotoxicity of preservatives used in cosmetic industry determined on consecutive passages of senescent diploid human lung fibroblasts in vitro
Biography
Biography: Katarzyna Miranowicz-Dzierżawska
Abstract
Statement of the Problem: Cell culture system could be a useful model for aging-related changes. The aim of the study was to assess whether there are differences between the results of determination of preservatives cytotoxicity obtained on senescent cells in different age.
Methodology & Theoretical Orientation: Experiments were conducted to determine the cytotoxicity of four preservatives: methylparaben, propylparaben, 2-phenoxyethanol and benzalkonium chloride on subsequent passages of senescent human lung CCD-8Lu (ATCC CCL-201TM) fibroblasts. The tests were carried out in passages no. 10 / 18. Xenobiotics cytotoxicity was evaluated using two cell viability assays: MTT assay, determining metabolic activity of cells, and NRU assay, assessing the integrity of cell membranes. The IC50 values were used as the main measure for comparing the cytotoxicity of tested compounds.
Findings: The results showed that the preservatives can be ranked according to the increasing cytotoxic potency towards the tested human diploid lung fibroblasts: 2-phenoxyethanol methylparaben propylparaben benzalkonium chloride. Older cells became less susceptible then the younger ones with cytotoxic effects of the xenobiotics tested in the majority of cases.
Conclusion & Significance: The passage number of diploid human lung fibroblasts had an important impact on the susceptibility of cells to preservatives. The test of the integrity of cell membranes (NRU) seems to be more appropriate to assess the cytotoxic effect of the investigated preservatives on diploid fibroblasts in different ages, which may be related to the mechanism of action of these compounds.
Funding: This paper has been based on the results of a research task I.N.13 carried out within the scope of the fourth stage of the National Programme Improvement of safety and working conditions partly supported in 2017–2019 — within the scope of research and development — by the Ministry of Science and Higher Education/National Centre for Research and Development.