Mohammed Al Sibani
Martin Luther University Halle-Wittenberg, Germany
Title: Evaluation of in vitro degradation rate of hyaluronic acid-based hydrogel cross-linked with 1, 4-butanediol diglycidyl ether (BDDE) using RP-HPLC and UV-Vis spectroscopy
Biography
Biography: Mohammed Al Sibani
Abstract
A hyaluronic acid (HA) was cross-linked with 1, 4-butanediol diglycidyl ether to produce nine BDDE-HA hydrogels. The degradation rates of six hydrogels were evaluated by HPLC and UV-Visible spectroscopy. The percentage amount of N-acetyl glucosamine (NAG) obtained after one-day enzymatic digestion to the total amount obtained after complete digestion was an indicative of the degradation rate of each hydrogel. The results were calculated with 95% confidence interval and showed (62.6%±12.3 w/w), precision value % R.S.D=7.95, average recovery=81.0%, LOD=6.4 µg/ml for HPLC and (63.3±13.9 w/w), precision value %R.S.D=8.83, average recovery=83.1%, LOD=5.4 µg/ml for UV method. The two methods showed also good linearity with correlation coefficients (R2) of 0.998 and 0.9995 for HPLC and UV method, respectively. For a comparison purpose, the other three hydrogels were rated using the conventional weight loss method which showed relatively higher degradation rates with an average of (73.4%±5.7 w/w), %R.S.D=3.13. Statistical analysis revealed that there was no significant difference between HPLC and UV-Visible methods, however, these values differed significantly (p<0.05) from the value obtained from the weight loss method.